Hotspot

clpP-psbB

Genome · 74724-75598 · 875 bp

Back to Callerya

Pi 0.00229

MarkerSeek score 60.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 875
variable_sites: 3
pi: 0.00229
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	GATGCTTCATCGTCTCCAGG	TGGACAGAAAGTAAACGGCC	55.0	50.0	58.1	58.1	999 bp	1.0	78.3
clpP-psbB_p2	GATGCTTCATCGTCTCCAGG	AAACGGCCGGGATCATTTAA	55.0	45.0	58.1	57.9	987 bp	1.0	78.2
clpP-psbB_p3	ATCGTCTCCAGGAACTCGAA	TGGACAGAAAGTAAACGGCC	50.0	50.0	58.2	58.1	991 bp	1.0	78.1
clpP-psbB_p4	ATCGTCTCCAGGAACTCGAA	AAACGGCCGGGATCATTTAA	50.0	45.0	58.2	57.9	979 bp	1.0	78.0
clpP-psbB_p5	GATGCTTCATCGTCTCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.1	58.2	951 bp	1.0	77.3