Hotspot

petL-petG

Genome · 69199-69361 · 163 bp

Back to Callerya

Pi 0.00818

MarkerSeek score 44.70

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: Genome
length_bp: 163
variable_sites: 2
pi: 0.00818
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	AGTCTGAATAAGATACGACTTATTTGA	GCAGTTACAAATAATCCAGCCA	29.6	40.9	56.5	57.0	264 bp	1.0	40.6
petL-petG_p2	AGTCTGAATAAGATACGACTTATTTGA	TGCAGTTACAAATAATCCAGCC	29.6	40.9	56.5	57.0	265 bp	1.0	40.6
petL-petG_p3	AGTCTGAATAAGATACGACTTAT	GCAGTTACAAATAATCCAGCCA	30.4	40.9	52.3	57.0	264 bp	1.0	40.6
petL-petG_p4	AGTCTGAATAAGATACGACTTAT	TGCAGTTACAAATAATCCAGCC	30.4	40.9	52.3	57.0	265 bp	1.0	40.6
petL-petG_p5	GTCTGAATAAGATACGACTTATTTGA	GCAGTTACAAATAATCCAGCCA	30.8	40.9	55.3	57.0	263 bp	1.0	40.6