Hotspot

atpI-atpH

Genome · 48873-50306 · 1434 bp

Back to Callerya

Pi 0.00209

MarkerSeek score 59.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: Genome
length_bp: 1434
variable_sites: 3
pi: 0.00209
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	GCTGCGGCTTATATAGGTGA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.3	58.0	1595-1596 bp	1.0	78.0
atpI-atpH_p2	ACAAGCGGTATTCAAGCTCT	CGCAATCCCTTCTACAGCTT	45.0	50.0	57.2	58.0	1631-1632 bp	1.0	77.7
atpI-atpH_p3	GGTGAATCCATGGAAGGTCA	CGCAATCCCTTCTACAGCTT	50.0	50.0	57.2	58.0	1580-1581 bp	1.0	77.5
atpI-atpH_p4	GCTGCGGCTTATATAGGTGA	AACAGAAGCGGCAGAAATCA	50.0	45.0	57.3	58.1	1505-1506 bp	1.0	77.4
atpI-atpH_p5	ACAAGCGGTATTCAAGCTCT	AACAGAAGCGGCAGAAATCA	45.0	45.0	57.2	58.1	1541-1542 bp	1.0	77.2