Hotspot

trnT-GGU-trnE-UUC

Genome · 31531-32339 · 809 bp

Back to Callerya

Pi 0.00124

MarkerSeek score 59.40

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: Genome
length_bp: 809
variable_sites: 1
pi: 0.00124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	TTTCGTAGTACCCTACCCCC	50.0	55.0	58.0	57.8	949-950 bp	1.0	82.8
trnT-GGU-trnE-UUC_p2	GGCGTTACTCTACCACACTG	TTTCGTAGTACCCTACCCCC	55.0	55.0	58.0	57.8	924-925 bp	1.0	82.8
trnT-GGU-trnE-UUC_p3	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	58.0	57.8	912-913 bp	1.0	82.7
trnT-GGU-trnE-UUC_p4	GGCGTTACTCTACCACACTG	CGTTGCCTCCTTGAAAGAGA	55.0	50.0	58.0	57.8	887-888 bp	1.0	82.7
trnT-GGU-trnE-UUC_p5	CCATGGCGTTACTCTACCAC	TTTCGTAGTACCCTACCCCC	55.0	55.0	58.1	57.8	928-929 bp	1.0	82.3