Hotspot

ndhD

SSC · 113363-114745 · 1383 bp

Back to Calendula

Pi 0.00217

MarkerSeek score 57.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1383
variable_sites: 3
pi: 0.00217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCCGCTCGACTCTTCAAAAT	GGAAATAGGGTAATCCGGTGG	50.0	52.4	58.9	57.9	1468 bp	1.0	63.7
ndhD_p2	CCGCTCGACTCTTCAAAATC	GGAAATAGGGTAATCCGGTGG	50.0	52.4	56.9	57.9	1467 bp	1.0	62.5
ndhD_p3	GCCGCTCGACTCTTCAAAAT	AGGGTAATCCGGTGGTATACT	50.0	47.6	58.9	57.4	1462 bp	1.0	59.8
ndhD_p4	TTTTTCATTCGCCGCTCGA	GGAAATAGGGTAATCCGGTGG	47.4	52.4	58.5	57.9	1478 bp	1.0	59.3
ndhD_p5	GCCGCTCGACTCTTCAAAA	GGAAATAGGGTAATCCGGTGG	52.6	52.4	58.5	57.9	1468 bp	1.0	59.3