Hotspot

ycf15-rps12

IRa · 136014-136958 · 945 bp

Back to Calendula

Pi 0.00317

MarkerSeek score 60.60

Primer available yes

Label ycf15-rps12

Feature Metadata

label_name: ycf15-rps12
feature_type: igs
region: IRa
length_bp: 945
variable_sites: 3
pi: 0.00317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-rps12_p1	GAAAGGACTCACTAAGCCGG	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.0	57.7	1020-1040 bp	1.0	78.1
ycf15-rps12_p2	CGGTACAATCCTCTTCCTGC	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.1	57.7	1107-1127 bp	1.0	77.7
ycf15-rps12_p3	GTACAATCCTCTTCCTGCCG	TGGCAACTTTACGTAAGGCA	55.0	45.0	58.1	57.7	1105-1125 bp	1.0	77.7
ycf15-rps12_p4	TACAATCCTCTTCCTGCCGA	TGGCAACTTTACGTAAGGCA	50.0	45.0	58.1	57.7	1104-1124 bp	1.0	77.0
ycf15-rps12_p5	GGTACAATCCTCTTCCTGCC	TGGCAACTTTACGTAAGGCA	55.0	45.0	57.7	57.7	1106-1126 bp	1.0	75.5