Hotspot

rps15-trnN-GUU

SSC · 121118-126836 · 5719 bp

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Pi 0.00542
MarkerSeek score 55.70
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
5719
variable_sites
31
pi
0.00542
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 TCTTGTTCTCCTTGCAAAAGA GAATCTACCCGTCTCTCTGT 38.1 50.0 55.6 55.8 307 bp 1.0 41.0
rps15-trnN-GUU_p2 TCTTGTTCTCCTTGCAAAAGAA GAATCTACCCGTCTCTCTGT 36.4 50.0 56.4 55.8 307 bp 1.0 41.0
rps15-trnN-GUU_p3 ATCTTGTTCTCCTTGCAAAAGA GAATCTACCCGTCTCTCTGT 36.4 50.0 56.2 55.8 308 bp 1.0 41.0
rps15-trnN-GUU_p4 CTTGTTCTCCTTGCAAAAGA GAATCTACCCGTCTCTCTGT 40.0 50.0 54.0 55.8 306 bp 1.0 41.0
rps15-trnN-GUU_p5 TCTTGTTCTCCTTGCAAAAG GAATCTACCCGTCTCTCTGT 40.0 50.0 54.0 55.8 307 bp 1.0 41.0