Hotspot

rpl32-trnL-UAG

SSC · 111242-111926 · 685 bp

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Pi 0.00584
MarkerSeek score 57.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
685
variable_sites
4
pi
0.00584
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TATAGGGCGGCCTTAAAAGC AGCGTGTCTACCGATTTCAC 50.0 50.0 57.7 58.0 807 bp 1.0 78.2
rpl32-trnL-UAG_p2 TATAGGGCGGCCTTAAAAGC CAGCGTGTCTACCGATTTCA 50.0 50.0 57.7 58.0 808 bp 1.0 78.2
rpl32-trnL-UAG_p3 GCGGCCTTAAAAGCTTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 801 bp 1.0 77.9
rpl32-trnL-UAG_p4 GCGGCCTTAAAAGCTTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 802 bp 1.0 77.9
rpl32-trnL-UAG_p5 AGGATATAGGGCGGCCTTAA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.7 58.0 811 bp 1.0 77.6