Hotspot

trnQ-UUG-rps16

LSC · 76211-77059 · 849 bp

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Pi 0.00361
MarkerSeek score 62.90
Primer available yes
Label trnQ-UUG-rps16

Feature Metadata

label_name
trnQ-UUG-rps16
feature_type
igs
region
LSC
length_bp
849
variable_sites
3
pi
0.00361
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-rps16_p1 GAGGTTCGAATCCTTCCGTC AAGTCGCACGTTGCTTTCTA 55.0 45.0 58.1 58.1 909-922 bp 1.0 80.1
trnQ-UUG-rps16_p2 GAGGTTCGAATCCTTCCGTC TCATGTCCTTCAAGTCGCAC 55.0 50.0 58.1 58.2 920-933 bp 1.0 79.6
trnQ-UUG-rps16_p3 GAGGTTCGAATCCTTCCGTC CACACCGGATCATGTCCTTC 55.0 55.0 58.1 58.3 929-942 bp 1.0 78.4
trnQ-UUG-rps16_p4 GAGGTTCGAATCCTTCCGTC CACCGGATCATGTCCTTCAA 55.0 50.0 58.1 57.6 927-940 bp 1.0 78.0
trnQ-UUG-rps16_p5 GAGGTTCGAATCCTTCCGTC CCGGATCATGTCCTTCAAGT 55.0 50.0 58.1 57.3 925-938 bp 1.0 75.6