Hotspot

trnD-GUC-psbM

LSC · 72004-72633 · 630 bp

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Pi 0.00635

MarkerSeek score 62.40

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 4
pi: 0.00635
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATAGGCAAGAGCACCG	AGTAGAAAGGCAGTAGGAACT	50.0	42.9	58.3	55.7	762 bp	1.0	52.0
trnD-GUC-psbM_p2	TTCAATAGGCAAGAGCACCG	GGAACTAAAATGAACAGTGCAGT	50.0	39.1	58.3	57.6	747 bp	1.0	50.8
trnD-GUC-psbM_p3	TTCAATAGGCAAGAGCACCG	AAAATGAACAGTGCAGTAGC	50.0	40.0	58.3	54.5	741 bp	1.0	50.4
trnD-GUC-psbM_p4	TTCAATAGGCAAGAGCACCG	AAGTAGAAAGGCAGTAGGAACT	50.0	40.9	58.3	56.5	763 bp	1.0	50.1
trnD-GUC-psbM_p5	TTCAATAGGCAAGAGCACCG	ACTAAAATGAACAGTGCAGTAGC	50.0	39.1	58.3	57.4	744 bp	1.0	49.4