Hotspot

rpoB-trnE-UUC

LSC · 70574-71500 · 927 bp

Back to Calendula

Pi 0.00217
MarkerSeek score 60.70
Primer available yes
Label rpoB-trnE-UUC

Feature Metadata

label_name
rpoB-trnE-UUC
feature_type
igs
region
LSC
length_bp
927
variable_sites
2
pi
0.00217
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnE-UUC_p1 AGTTCCTCTGTCAAACCTTGA GGGACTTGAACCCTCGTTTT 42.9 50.0 56.8 58.0 1069 bp 1.0 62.8
rpoB-trnE-UUC_p2 AGTTCCTCTGTCAAACCTTGA GAGTTTTGGTACACCCCCAG 42.9 55.0 56.8 58.1 1090 bp 1.0 62.2
rpoB-trnE-UUC_p3 AGTTCCTCTGTCAAACCTTGA GTTTTGGTACACCCCCAGAG 42.9 55.0 56.8 58.1 1088 bp 1.0 62.2
rpoB-trnE-UUC_p4 AGTTCCTCTGTCAAACCTTGA TAACCACTGGACCATAGGGG 42.9 55.0 56.8 58.1 1028 bp 1.0 62.1
rpoB-trnE-UUC_p5 AGTTCCTCTGTCAAACCTTGA CCCTCGTTTTCTCCGTGAAA 42.9 50.0 56.8 58.1 1059 bp 1.0 62.0