Hotspot

trnL-UAA-trnT-UGU

LSC · 36696-37225 · 530 bp

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Pi 0.0076
MarkerSeek score 62.40
Primer available yes
Label trnL-UAA-trnT-UGU

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnT-UGU_p1 ACCAATTTCGCCATATCCCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.0 57.9 576-580 bp 1.0 80.0
trnL-UAA-trnT-UGU_p2 TAGCGTCTACCAATTTCGCC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.1 57.9 584-588 bp 1.0 79.5
trnL-UAA-trnT-UGU_p3 ACCAATTTCGCCATATCCCC GGAATCGAACCGATGACCAT 50.0 50.0 58.0 57.8 608-612 bp 1.0 79.1
trnL-UAA-trnT-UGU_p4 TAGCGTCTACCAATTTCGCC GGAATCGAACCGATGACCAT 50.0 50.0 58.1 57.8 616-620 bp 1.0 78.6
trnL-UAA-trnT-UGU_p5 AGTCCGTAGCGTCTACCAAT GCGATGCTCTAACCTCTGAG 50.0 55.0 58.2 57.9 590-594 bp 1.0 78.1