Hotspot

trnV-UAC-ndhC

LSC · 32476-33464 · 989 bp

Back to Calendula

Pi 0.00508

MarkerSeek score 62.20

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 989
variable_sites: 5
pi: 0.00508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	AGAAGGTCTACGGTTCGAGT	GTCCTTTGCTAATCGGAGCT	50.0	50.0	57.8	57.7	1134-1154 bp	1.0	76.6
trnV-UAC-ndhC_p2	AGAAGGTCTACGGTTCGAGT	CGGTCCTTTGCTAATCGGAG	50.0	55.0	57.8	58.4	1136-1156 bp	1.0	75.7
trnV-UAC-ndhC_p3	AGAAGGTCTACGGTTCGAGT	CTCCGGTCCTTTGCTAATCG	50.0	55.0	57.8	58.4	1139-1159 bp	1.0	75.7
trnV-UAC-ndhC_p4	GAAGGTCTACGGTTCGAGTC	GTCCTTTGCTAATCGGAGCT	55.0	50.0	57.5	57.7	1133-1153 bp	1.0	73.8
trnV-UAC-ndhC_p5	AGAAGGTCTACGGTTCGAGT	GGTCCTTTGCTAATCGGAGC	50.0	55.0	57.8	58.7	1135-1155 bp	1.0	73.5