Hotspot

psbJ-petA

LSC · 20068-20851 · 784 bp

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Pi 0.00515

MarkerSeek score 62.60

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 4
pi: 0.00515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	TGTGATCGGTTTAGTAGGCG	ACGTGTACAAGGCCTTTTGT	50.0	45.0	57.4	58.0	953-960 bp	1.0	76.0
psbJ-petA_p2	GTGATCGGTTTAGTAGGCGT	ACGTGTACAAGGCCTTTTGT	50.0	45.0	57.4	58.0	952-959 bp	1.0	76.0
psbJ-petA_p3	TGTGATCGGTTTAGTAGGCG	GGCCTTTTGTTCTTCTTCGC	50.0	50.0	57.4	57.9	943-950 bp	1.0	75.9
psbJ-petA_p4	GTGATCGGTTTAGTAGGCGT	GGCCTTTTGTTCTTCTTCGC	50.0	50.0	57.4	57.9	942-949 bp	1.0	75.9
psbJ-petA_p5	TATTCCGGATTGGGTTCGTC	ACGTGTACAAGGCCTTTTGT	50.0	45.0	57.4	58.0	916-923 bp	1.0	75.7