Hotspot

petG-petL

LSC · 17768-17932 · 165 bp

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Pi 0.0182

MarkerSeek score 63.00

Primer available yes

Label petG-petL

Feature Metadata

label_name: petG-petL
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 3
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-petL_p1	TCAAAGGTCCAACTGATCGC	TAGCTCCTTGATGCCTACCA	50.0	50.0	58.2	57.8	385 bp	1.0	77.7
petG-petL_p2	TCAAAGGTCCAACTGATCGC	AGCTCCTTGATGCCTACCAT	50.0	50.0	58.2	58.5	384 bp	1.0	75.2
petG-petL_p3	CGCCACGCCTGTATTGTAAA	TAGCTCCTTGATGCCTACCA	50.0	50.0	58.9	57.8	368 bp	1.0	71.9
petG-petL_p4	TCAAAGGTCCAACTGATCGC	TGGTCTGAGCAAGATACGAC	50.0	50.0	58.2	57.1	307 bp	1.0	71.7
petG-petL_p5	TCAAAGGTCCAACTGATCGC	AGCTCCTTGATGCCTACCA	50.0	52.6	58.2	58.0	384 bp	1.0	71.0