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rps12-rpl20

LSC · 14312-15044 · 733 bp

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Pi 0.00546

MarkerSeek score 61.90

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 4
pi: 0.00546
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	CAGCGTCGAGGAACATGTAC	TCTCCGAGCGATATATCCCC	55.0	55.0	58.7	57.9	799 bp	1.0	74.1
rps12-rpl20_p2	AGCGTCGAGGAACATGTACT	TCTCCGAGCGATATATCCCC	50.0	55.0	58.8	57.9	798 bp	1.0	73.4
rps12-rpl20_p3	TCAGCGTCGAGGAACATGTA	TCTCCGAGCGATATATCCCC	50.0	55.0	58.8	57.9	800 bp	1.0	73.3
rps12-rpl20_p4	GCGTCGAGGAACATGTACTA	TCTCCGAGCGATATATCCCC	50.0	55.0	57.2	57.9	797 bp	1.0	73.3
rps12-rpl20_p5	CAGCGTCGAGGAACATGTAC	TTCTACGTCTCCGAGCGATA	55.0	50.0	58.7	57.8	806 bp	1.0	72.7