Hotspot

rps15

IRb · 124906-125178 · 273 bp

Back to Caldesia

Pi 0.00366

MarkerSeek score 43.20

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: IRb
length_bp: 273
variable_sites: 1
pi: 0.00366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GAAAACAACGTAAAGGGCCG	TGGTTAACTCCAGCGGAAAG	50.0	50.0	58.0	57.8	1491 bp	0.5	58.2
rps15_p2	CAAGCGGATCTCGAAATGGA	TGGTTAACTCCAGCGGAAAG	50.0	50.0	58.1	57.8	1255 bp	0.5	58.1
rps15_p3	TCAAGCGGATCTCGAAATGG	TGGTTAACTCCAGCGGAAAG	50.0	50.0	58.1	57.8	1256 bp	0.5	58.1
rps15_p4	AGGGCCGTGGACAATTTATG	TGGTTAACTCCAGCGGAAAG	50.0	50.0	58.2	57.8	1478 bp	0.5	56.7
rps15_p5	GGGCCGTGGACAATTTATGA	TGGTTAACTCCAGCGGAAAG	50.0	50.0	58.2	57.8	1477 bp	0.5	56.6