Hotspot

accD

LSC · 63203-64474 · 1272 bp

Back to Caldesia

Pi 0.00865

MarkerSeek score 51.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1272
variable_sites: 11
pi: 0.00865
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACACGCTTGTTAGGTCGATC	TGTTACCAAGGAAAACGCCA	50.0	45.0	58.0	57.9	2716-2767 bp	1.0	81.6
accD_p2	TCTTGACACGGGCACTTTTT	TGTTACCAAGGAAAACGCCA	45.0	45.0	58.2	57.9	2056-2062 bp	1.0	78.7
accD_p3	TAGTCTTGACACGGGCACTT	TGTTACCAAGGAAAACGCCA	50.0	45.0	59.0	57.9	2059-2065 bp	1.0	73.0
accD_p4	GTCTTGACACGGGCACTTTT	TGTTACCAAGGAAAACGCCA	50.0	45.0	59.0	57.9	2057-2063 bp	1.0	72.8
accD_p5	TAGTCTTGACACGGGCACT	TGTTACCAAGGAAAACGCCA	52.6	45.0	58.3	57.9	2059-2065 bp	1.0	70.5