Hotspot

trnN-GUU-rps15

IRb · 115091-124905 · 9815 bp

Back to Caldesia

Pi 0.00341
MarkerSeek score 53.50
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
IRb
length_bp
9815
variable_sites
25
pi
0.00341
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.75
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 CAAGCGGATCTCGAAATGGA GGAATTGCTGAGATGAATGCA 50.0 42.9 58.1 56.9 356 bp 1.0 63.9
trnN-GUU-rps15_p2 TCAAGCGGATCTCGAAATGG GGAATTGCTGAGATGAATGCA 50.0 42.9 58.1 56.9 357 bp 1.0 63.9
trnN-GUU-rps15_p3 CAAGCGGATCTCGAAATGGA GGAATTGCTGAGATGAATGCAT 50.0 40.9 58.1 57.4 356 bp 1.0 59.7
trnN-GUU-rps15_p4 TCAAGCGGATCTCGAAATGG GGAATTGCTGAGATGAATGCAT 50.0 40.9 58.1 57.4 357 bp 1.0 59.7
trnN-GUU-rps15_p5 AAGCGGATCTCGAAATGGAA GGAATTGCTGAGATGAATGCA 45.0 42.9 57.3 56.9 355 bp 1.0 58.8