Hotspot

rpl16-rps3

LSC · 87638-88858 · 1221 bp

Back to Caldesia

Pi 0.00657

MarkerSeek score 62.80

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 8
pi: 0.00657
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TCCGAAACAAACACGATTGC	GACGGTAGAGTTCCCCTACA	45.0	55.0	57.6	57.9	1429-1431 bp	1.0	76.4
rpl16-rps3_p2	CGAAACAAACACGATTGCCT	GACGGTAGAGTTCCCCTACA	45.0	55.0	57.6	57.9	1427-1429 bp	1.0	76.3
rpl16-rps3_p3	GTTCAAGTGCCTGAAGAGCA	GACGGTAGAGTTCCCCTACA	50.0	55.0	58.4	57.9	1454-1456 bp	1.0	76.3
rpl16-rps3_p4	GGGTTCAAGTGCCTGAAGAG	GACGGTAGAGTTCCCCTACA	55.0	55.0	58.5	57.9	1456-1458 bp	1.0	75.8
rpl16-rps3_p5	TTCAAGTGCCTGAAGAGCAT	GACGGTAGAGTTCCCCTACA	45.0	55.0	57.4	57.9	1453-1455 bp	1.0	74.9