Hotspot

petL-petG

LSC · 71568-71743 · 176 bp

Back to Caldesia

Pi 0.00568

MarkerSeek score 50.00

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 1
pi: 0.00568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTTTTCTACTGGCTGCTT	ATCCAACTGATCACCACGTC	45.0	50.0	57.3	57.6	357 bp	1.0	71.9
petL-petG_p2	TTGGTTTTCTACTGGCTGCT	ATCCAACTGATCACCACGTC	45.0	50.0	57.3	57.6	358 bp	1.0	71.9
petL-petG_p3	TGGTTTTCTACTGGCTGCTT	TCCAACTGATCACCACGTCT	45.0	50.0	57.3	58.7	356 bp	1.0	69.7
petL-petG_p4	TTGGTTTTCTACTGGCTGCT	TCCAACTGATCACCACGTCT	45.0	50.0	57.3	58.7	357 bp	1.0	69.7
petL-petG_p5	TTGGTTTTCTACTGGCTGCTT	ATCCAACTGATCACCACGTC	42.9	50.0	58.1	57.6	358 bp	1.0	68.7