Hotspot

ycf4-cemA

LSC · 66463-67365 · 903 bp

Back to Caldesia

Pi 0.00332

MarkerSeek score 60.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 3
pi: 0.00332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCGATTCCTTTGACTCGT	AGAGAGATAGACCAGGGCAA	50.0	50.0	58.0	57.1	1084-1085 bp	1.0	73.3
ycf4-cemA_p2	CTCCGCGAGAGATTGAACAA	AGAGAGATAGACCAGGGCAA	50.0	50.0	58.0	57.1	1048-1049 bp	1.0	73.3
ycf4-cemA_p3	TGAGAATTTGACTCCGCGAG	AGAGAGATAGACCAGGGCAA	50.0	50.0	58.0	57.1	1059-1060 bp	1.0	73.3
ycf4-cemA_p4	TTTGACTCCGCGAGAGATTG	AGAGAGATAGACCAGGGCAA	50.0	50.0	58.0	57.1	1053-1054 bp	1.0	73.3
ycf4-cemA_p5	TTAGCCTATTTCTTGCGCGT	AGAGAGATAGACCAGGGCAA	45.0	50.0	58.0	57.1	1016-1017 bp	1.0	73.2