Hotspot

ycf3-trnS-GGA

LSC · 48264-48838 · 575 bp

Back to Caldesia

Pi 0.00873
MarkerSeek score 63.60
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
575
variable_sites
5
pi
0.00873
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTTTCTCCGGAAGTTGTCGG CTACACCTTCAACCACTCGG 50.0 55.0 58.1 57.9 701-703 bp 1.0 79.1
ycf3-trnS-GGA_p2 TTTTCTCCGGAAGTTGTCGG CCCTCGGTAAACAAAAGCCT 50.0 50.0 58.1 58.1 738-740 bp 1.0 79.0
ycf3-trnS-GGA_p3 TGCCTCTTTTTCTCCGGAAG CTACACCTTCAACCACTCGG 50.0 55.0 57.8 57.9 708-710 bp 1.0 78.7
ycf3-trnS-GGA_p4 GCCTCTTTTTCTCCGGAAGT CTACACCTTCAACCACTCGG 50.0 55.0 57.8 57.9 707-709 bp 1.0 78.7
ycf3-trnS-GGA_p5 TTTTCTCCGGAAGTTGTCGG ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.1 58.2 759-761 bp 1.0 78.5