Hotspot

trnM-CAU-psbD

LSC · 35367-36369 · 1003 bp

Back to Caldesia

Pi 0.00698
MarkerSeek score 62.80
Primer available yes
Label trnM-CAU-psbD

Feature Metadata

label_name
trnM-CAU-psbD
feature_type
igs
region
LSC
length_bp
1003
variable_sites
7
pi
0.00698
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-psbD_p1 GTGGTAGAGTAACGCCATGG CACAAAAACGAAACGGTCCC 55.0 50.0 58.1 58.2 1147-1153 bp 1.0 78.6
trnM-CAU-psbD_p2 GTGGTAGAGTAACGCCATGG CCACAAAAACGAAACGGTCC 55.0 50.0 58.1 58.2 1148-1154 bp 1.0 78.6
trnM-CAU-psbD_p3 GTGGTAGAGTAACGCCATGG CCCACAAAAACGAAACGGTC 55.0 50.0 58.1 58.2 1149-1155 bp 1.0 78.6
trnM-CAU-psbD_p4 TCAGTGGTAGAGTAACGCCA CACAAAAACGAAACGGTCCC 50.0 50.0 58.1 58.2 1150-1156 bp 1.0 78.4
trnM-CAU-psbD_p5 TCAGTGGTAGAGTAACGCCA CCACAAAAACGAAACGGTCC 50.0 50.0 58.1 58.2 1151-1157 bp 1.0 78.4