Hotspot

atpH-atpI

LSC · 15939-16909 · 971 bp

Back to Caldesia

Pi 0.00721

MarkerSeek score 59.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 7
pi: 0.00721
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1044-1045 bp	1.0	74.3
atpH-atpI_p2	CAGTACCTTGACCAACTCCG	CCGCGGCTTATATAGGTGAA	55.0	50.0	57.9	57.3	1103-1104 bp	1.0	74.0
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGC	45.0	55.0	58.0	58.9	1032-1033 bp	1.0	73.4
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.2	57.3	1045-1046 bp	1.0	73.2
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	CCGCGGCTTATATAGGTGAA	55.0	50.0	58.2	57.3	1104-1105 bp	1.0	73.1