Hotspot

ndhF-rpl32

SSC · 115523-116541 · 1019 bp

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Pi 0.0251

MarkerSeek score 69.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1019
variable_sites: 160
pi: 0.0251
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAAAAGGGGTATTATCCATGC	AGAAAAAGCTTTTACTGCCTCT	43.5	36.4	57.5	56.2	829-1258 bp	1.0	52.0
ndhF-rpl32_p2	GGAAAAAGGGGTATTATCCATGC	AAGAAAAAGCTTTTACTGCCTCT	43.5	34.8	57.5	56.9	830-1259 bp	1.0	52.0
ndhF-rpl32_p3	GGAAAAAGGGGTATTATCCATGC	GAAAAAGCTTTTACTGCCTCT	43.5	38.1	57.5	54.7	828-1257 bp	1.0	52.0
ndhF-rpl32_p4	GGAAAAAGGGGTATTATCCATGC	GCTTTTACTGCCTCTAAAGATCC	43.5	43.5	57.5	57.3	822-1251 bp	0.973684	50.9
ndhF-rpl32_p5	AAAGGGGTATTATCCATGCA	GCTTTTACTGCCTCTAAAGATCC	40.0	43.5	54.0	57.3	818-1247 bp	0.973684	50.9