Hotspot

psbB-psbT

LSC · 76312-76918 · 607 bp

Back to Calanthe

Pi 0.0344

MarkerSeek score 72.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 607
variable_sites: 105
pi: 0.0344
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	ACAAGGAAACAAGTAGTCTGA	TGGAACTTTAGGTGGTTCTCG	38.1	47.6	54.4	57.6	668-837 bp	0.947368	49.9
psbB-psbT_p2	ACAAGGAAACAAGTAGTCTGA	GGAACTTTAGGTGGTTCTCGA	38.1	47.6	54.4	57.3	667-836 bp	0.947368	49.9
psbB-psbT_p3	ACAAGGAAACAAGTAGTCTGA	GGAACTTTAGGTGGTTCTCGAA	38.1	45.5	54.4	58.0	667-836 bp	0.947368	49.9
psbB-psbT_p4	ACAAGGAAACAAGTAGTCTG	TGGAACTTTAGGTGGTTCTCG	40.0	47.6	52.8	57.6	668-837 bp	0.947368	49.9
psbB-psbT_p5	ACAAGGAAACAAGTAGTCTGA	GGAACTTTAGGTGGTTCTCG	38.1	50.0	54.4	55.8	667-836 bp	0.947368	49.9