Hotspot

ycf4-cemA

LSC · 62263-63030 · 768 bp

Back to Calanthe

Pi 0.0193
MarkerSeek score 70.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
768
variable_sites
95
pi
0.0193
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 ACAAAAAGCTGCCGAATTGG ATCCACCAGGGCAAAAAGAG 45.0 50.0 57.8 58.1 776-920 bp 1.0 90.2
ycf4-cemA_p2 ATTCCCTTGACCCGTACTGA ATCCACCAGGGCAAAAAGAG 50.0 50.0 58.1 58.1 823-967 bp 0.947368 88.9
ycf4-cemA_p3 TTCCCTTGACCCGTACTGAT ATCCACCAGGGCAAAAAGAG 50.0 50.0 58.1 58.1 822-966 bp 0.947368 88.9
ycf4-cemA_p4 ATTCCCTTGACCCGTACTGA GAGAAATCCACCAGGGCAAA 50.0 50.0 58.1 58.1 828-972 bp 0.947368 88.9
ycf4-cemA_p5 TTCCCTTGACCCGTACTGAT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.1 58.1 827-971 bp 0.947368 88.9