Hotspot

ndhC-trnV-UAC

LSC · 51198-52606 · 1409 bp

Back to Calanthe

Pi 0.0208

MarkerSeek score 68.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1409
variable_sites: 152
pi: 0.0208
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAATCCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1243-1681 bp	1.0	89.3
ndhC-trnV-UAC_p2	CGGGGCTAAAATCCCAGAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1242-1680 bp	1.0	86.6
ndhC-trnV-UAC_p3	TCTGGACCTTCCCTAATCGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	1260-1698 bp	0.947368	86.6
ndhC-trnV-UAC_p4	AATCGGGGCTAAAATCCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1246-1684 bp	0.947368	85.1
ndhC-trnV-UAC_p5	TCTGGACCTTCCCTAATCGG	GAAGGTCTACGGTTCGAGTC	55.0	55.0	58.2	57.5	1259-1697 bp	0.947368	83.9