Hotspot

trnE-UUC-trnT-GGU

LSC · 32399-33227 · 829 bp

Back to Calanthe

Pi 0.0278

MarkerSeek score 68.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 121
pi: 0.0278
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	668-949 bp	1.0	90.1
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	665-946 bp	1.0	90.0
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	646-927 bp	1.0	89.2
trnE-UUC-trnT-GGU_p4	ATGACCAACTTCCTTTCGCA	CCATGGCGTTACTCTACCAC	45.0	55.0	57.7	58.1	718-999 bp	0.921053	86.2
trnE-UUC-trnT-GGU_p5	ATGACCAACTTCCTTTCGCA	TGGCGTTACTCTACCACTGA	45.0	50.0	57.7	58.1	715-996 bp	0.921053	86.0