Hotspot

petN-psbM

LSC · 29754-30596 · 843 bp

Back to Calanthe

Pi 0.0281

MarkerSeek score 67.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 116
pi: 0.0281
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGACATCATTCGCATGGAT	AAAGGAAAAGCGGAAAAGCG	50.0	45.0	58.1	57.9	931-1376 bp	0.789474	80.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	AAAGGAAAAGCGGAAAAGCG	50.0	45.0	58.2	57.9	910-1355 bp	0.789474	79.7
petN-psbM_p3	GACACTCCGTTTTGGCTACT	AAAGGAAAAGCGGAAAAGCG	50.0	45.0	57.8	57.9	1409-1830 bp	0.763158	77.5
petN-psbM_p4	GACTCCCTAGAGGCTCTGAG	ACGTCAAAACAGTCAGCCAA	60.0	45.0	58.0	58.3	823-1265 bp	0.447368	66.7
petN-psbM_p5	GACTCCCTAGAGGCTCTGAG	AAAGGAAAAGCGGAAAAGCG	60.0	45.0	58.0	57.9	1203-1624 bp	0.342105	61.1