Hotspot

trnC-GCA-petN

LSC · 29019-29657 · 639 bp

Back to Calanthe

Pi 0.0153

MarkerSeek score 64.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 55
pi: 0.0153
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CCAGTTCGAATCTGGGTGTC	GCTCTAAAGCCCACTCCTTC	55.0	55.0	58.3	58.0	487-940 bp	1.0	86.8
trnC-GCA-petN_p2	CCAGTTCGAATCTGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.3	58.2	421-874 bp	1.0	86.1
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.5	58.0	510-963 bp	1.0	84.6
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.5	58.2	444-897 bp	1.0	84.0
trnC-GCA-petN_p5	CAGTTCGAATCTGGGTGTCG	GCTCTAAAGCCCACTCCTTC	55.0	55.0	58.6	58.0	486-939 bp	1.0	83.8