Hotspot

rpoB-trnC-GCA

LSC · 27511-28937 · 1427 bp

Back to Calanthe

Pi 0.0196

MarkerSeek score 71.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1427
variable_sites: 153
pi: 0.0196
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1187-1643 bp	1.0	86.4
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	1211-1667 bp	1.0	85.4
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1224-1680 bp	1.0	84.5
rpoB-trnC-GCA_p4	AAATTCTTCCGTCAAGCCCT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.4	57.5	1188-1644 bp	1.0	83.1
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.2	58.0	1188-1644 bp	1.0	82.4