Hotspot

matK-trnK-UUU

LSC · 3317-4365 · 1049 bp

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Pi 0.0231

MarkerSeek score 66.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 123
pi: 0.0231
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGGAAGTTTTGTTGCCGAAA	ATCCGGGTTGCTAACTCAAC	40.0	50.0	56.4	57.9	993-1288 bp	1.0	78.1
matK-trnK-UUU_p2	AGGAAGTTTTGTTGCCGAAA	AACGGTAGAGTACTCGGCTT	40.0	50.0	56.4	58.2	976-1271 bp	1.0	77.6
matK-trnK-UUU_p3	AGGAAGTTTTGTTGCCGAAA	ACGGTAGAGTACTCGGCTTT	40.0	50.0	56.4	58.2	975-1270 bp	1.0	77.6
matK-trnK-UUU_p4	AGGAAGTTTTGTTGCCGAAA	GATCCGGGTTGCTAACTCAA	40.0	50.0	56.4	57.6	994-1289 bp	1.0	75.8
matK-trnK-UUU_p5	AGGAAGTTTTGTTGCCGAAA	TTGATCCGGGTTGCTAACTC	40.0	50.0	56.4	57.6	996-1291 bp	1.0	75.8