Hotspot

rps3

LSC · 84394-85050 · 657 bp

Back to Calamus

Pi 0.00913

MarkerSeek score 42.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 18
pi: 0.00913
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CTTCCGGCAAAATTCGTTCC	CCAATCTGACTTTCGCGAGA	50.0	50.0	58.0	58.0	1995-2051 bp	1.0	83.4
rps3_p2	CTTCCGGCAAAATTCGTTCC	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	1991-2047 bp	1.0	83.3
rps3_p3	CTGAACTCTGCAATGGAGCT	CCAATCTGACTTTCGCGAGA	50.0	50.0	57.9	58.0	2013-2069 bp	1.0	82.7
rps3_p4	CTTCCGGCAAAATTCGTTCC	ATTGGTGGGGGATGACCTTA	50.0	50.0	58.0	58.0	1810-1851 bp	1.0	82.4
rps3_p5	CTTCCGGCAAAATTCGTTCC	TTGGTGGGGGATGACCTTAT	50.0	50.0	58.0	58.0	1809-1850 bp	1.0	82.4