Hotspot

rps19

IRb · 85646-85924 · 279 bp

Back to Calamus

Pi 0.00119

MarkerSeek score 23.10

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: IRb
length_bp: 279
variable_sites: 1
pi: 0.00119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TAAGGTCATCCCCCACCAAT	GTTATCCTGCGCTTGGAAGA	50.0	50.0	58.0	58.0	760-777 bp	1.0	83.5
rps19_p2	ATAAGGTCATCCCCCACCAA	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	898-915 bp	1.0	83.1
rps19_p3	TAAGGTCATCCCCCACCAAT	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	897-914 bp	1.0	83.1
rps19_p4	ATAAGGTCATCCCCCACCAA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	899-916 bp	1.0	83.1
rps19_p5	TAAGGTCATCCCCCACCAAT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	898-915 bp	1.0	83.1