Hotspot

rps16

LSC · 4804-5949 · 1146 bp

Back to Calamus

Pi 0.00134

MarkerSeek score 58.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1146
variable_sites: 4
pi: 0.00134
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCAAGAAAAAGGCAAATTGTGT	ATACACACCGGTTGTTGAGC	36.4	50.0	57.1	58.5	1329 bp	0.166667	19.8
rps16_p2	ACAGCAAGAAAAAGGCAAATTG	ATACACACCGGTTGTTGAGC	36.4	50.0	56.9	58.5	1332 bp	0.166667	17.6
rps16_p3	AGCAAGAAAAAGGCAAATTGTG	ATACACACCGGTTGTTGAGC	36.4	50.0	56.9	58.5	1330 bp	0.166667	17.6
rps16_p4	CAGCAAGAAAAAGGCAAATTGT	ATACACACCGGTTGTTGAGC	36.4	50.0	56.9	58.5	1331 bp	0.166667	17.6
rps16_p5	CAGCAAGAAAAAGGCAAATTGTG	ATACACACCGGTTGTTGAGC	39.1	50.0	58.2	58.5	1331 bp	0.166667	17.5