Hotspot

psbA

LSC · 133-1194 · 1062 bp

Back to Calamus

Pi 0.00119

MarkerSeek score 39.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTCCATTGTTTGATTCAACG	TGGGAGCCCTTGAAAATTGA	40.9	45.0	58.1	57.3	1147 bp	1.0	57.9
psbA_p2	TGCTCCATTGTTTGATTCAACG	GCTTGGGAGCCCTTGAAAAT	40.9	50.0	58.1	58.7	1150 bp	1.0	57.6
psbA_p3	GCTCCATTGTTTGATTCAACGA	TGGGAGCCCTTGAAAATTGA	40.9	45.0	57.8	57.3	1146 bp	1.0	57.4
psbA_p4	GCTCCATTGTTTGATTCAACGA	GCTTGGGAGCCCTTGAAAAT	40.9	50.0	57.8	58.7	1149 bp	1.0	57.1
psbA_p5	TGCTCCATTGTTTGATTCAACG	TGGGAGCCCTTGAAAATTGAA	40.9	42.9	58.1	58.0	1147 bp	1.0	55.2