Hotspot

ccsA-ndhD

SSC · 117222-117446 · 225 bp

Back to Calamus

Pi 0.0186

MarkerSeek score 62.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 225
variable_sites: 9
pi: 0.0186
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCGGCACTTGTAGCT	TCAGTTGACAGGGTACAAGC	50.0	50.0	58.6	57.5	382-392 bp	1.0	74.5
ccsA-ndhD_p2	CGAATTCGGCACTTGTAGCT	CAGTTGACAGGGTACAAGCT	50.0	50.0	58.6	57.4	381-391 bp	1.0	74.4
ccsA-ndhD_p3	ATTCGGCACTTGTAGCTTCT	TCAGTTGACAGGGTACAAGC	45.0	50.0	57.2	57.5	379-389 bp	1.0	73.5
ccsA-ndhD_p4	ATTCGGCACTTGTAGCTTCT	CAGTTGACAGGGTACAAGCT	45.0	50.0	57.2	57.4	378-388 bp	1.0	73.4
ccsA-ndhD_p5	ACGAATTCGGCACTTGTAGC	TCAGTTGACAGGGTACAAGC	50.0	50.0	58.9	57.5	383-393 bp	1.0	72.2