Hotspot

rpl22-rps19

IRb · 85422-85645 · 224 bp

Back to Calamus

Pi 0.0347
MarkerSeek score 76.80
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
224
variable_sites
18
pi
0.0347
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CGTCCGCGAATCTGATCAAT CCAATCTGACTTTCGCGAGA 50.0 50.0 58.5 58.0 318-335 bp 1.0 84.0
rpl22-rps19_p2 CGTCCGCGAATCTGATCAAT TCTGACTTTCGCGAGACATG 50.0 50.0 58.5 58.0 314-331 bp 1.0 84.0
rpl22-rps19_p3 CATAGGAACGTCCGCGAATC CCAATCTGACTTTCGCGAGA 55.0 50.0 58.9 58.0 326-343 bp 1.0 80.9
rpl22-rps19_p4 CATAGGAACGTCCGCGAATC TCTGACTTTCGCGAGACATG 55.0 50.0 58.9 58.0 322-339 bp 1.0 80.9
rpl22-rps19_p5 CGTCCGCGAATCTGATCAAT CAAATTGGGAGAATTCGCGC 50.0 50.0 58.5 58.5 338-355 bp 1.0 79.9