Hotspot

rpl16-rps3

LSC · 84248-84393 · 146 bp

Back to Calamus

Pi 0.0365

MarkerSeek score 64.70

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 15
pi: 0.0365
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTTCGTCGTTCCCATAGCT	CATCGTTCCTTTTGGTTCGC	50.0	50.0	58.0	58.0	1357-1398 bp	1.0	82.9
rpl16-rps3_p2	CTTCCGGCAAAATTCGTTCC	CATCGTTCCTTTTGGTTCGC	50.0	50.0	58.0	58.0	1300-1341 bp	1.0	82.9
rpl16-rps3_p3	CTGAACTCTGCAATGGAGCT	CATCGTTCCTTTTGGTTCGC	50.0	50.0	57.9	58.0	1318-1359 bp	1.0	82.1
rpl16-rps3_p4	TCGTTCCCATAGCTTCTCCA	CATCGTTCCTTTTGGTTCGC	50.0	50.0	58.1	58.0	1351-1392 bp	1.0	81.8
rpl16-rps3_p5	AGTCACAGGTTTCGTCGTTC	CATCGTTCCTTTTGGTTCGC	50.0	50.0	58.2	58.0	1365-1406 bp	1.0	81.7