Hotspot

accD-psaI

LSC · 59772-60804 · 1033 bp

Back to Calamus

Pi 0.0272
MarkerSeek score 68.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
1033
variable_sites
57
pi
0.0272
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 CCACGGTTTCTTTCCCTTGA GGTCCCATCGGATCTAGACA 50.0 55.0 58.0 58.0 1156-1383 bp 1.0 87.8
accD-psaI_p2 CCACGGTTTCTTTCCCTTGA TGGTCCCATCGGATCTAGAC 50.0 55.0 58.0 58.0 1157-1384 bp 1.0 87.8
accD-psaI_p3 TCCACGGTTTCTTTCCCTTG GGTCCCATCGGATCTAGACA 50.0 55.0 58.0 58.0 1157-1384 bp 1.0 87.8
accD-psaI_p4 GACAGTACCCGATGGTTCAC GGTCCCATCGGATCTAGACA 55.0 55.0 58.0 58.0 1267-1494 bp 1.0 87.7
accD-psaI_p5 GACAGTACCCGATGGTTCAC TGGTCCCATCGGATCTAGAC 55.0 55.0 58.0 58.0 1268-1495 bp 1.0 87.7