Hotspot

rps3

LSC · 79443-80162 · 720 bp

Back to Calamagrostis

Pi 0.000694

MarkerSeek score 43.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 720
variable_sites: 1
pi: 0.000694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	CACTGGACAGAATGCGAAGA	TCATGTAAGATTTCAATTCCCACT	50.0	33.3	57.9	56.3	797 bp	1.0	40.1
rps3_p2	CTGGACAGAATGCGAAGACA	TCATGTAAGATTTCAATTCCCACT	50.0	33.3	57.9	56.3	795 bp	1.0	40.1
rps3_p3	TCACTGGACAGAATGCGAAG	TCATGTAAGATTTCAATTCCCACT	50.0	33.3	57.9	56.3	798 bp	1.0	40.1
rps3_p4	ACTGGACAGAATGCGAAGAC	TCATGTAAGATTTCAATTCCCACT	50.0	33.3	57.9	56.3	796 bp	1.0	40.1
rps3_p5	CTGGACAGAATGCGAAGACA	TTCATGTAAGATTTCAATTCCCACT	50.0	32.0	57.9	57.0	796 bp	1.0	40.1