Hotspot

ndhA

SSC · 111955-114067 · 2113 bp

Back to Calamagrostis

Pi 0.00379

MarkerSeek score 50.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2113
variable_sites: 14
pi: 0.00379
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	TGGGAGAAGTTGATCGTTGA	47.6	45.0	58.1	56.5	2253-2256 bp	1.0	59.8
ndhA_p2	ACCCAGTTACCATAGGGAACA	TGGGAGAAGTTGATCGTTGAA	47.6	42.9	58.1	57.2	2253-2256 bp	1.0	57.8
ndhA_p3	ACCCAGTTACCATAGGGAACA	ATGGGAGAAGTTGATCGTTGA	47.6	42.9	58.1	57.0	2254-2257 bp	1.0	56.0
ndhA_p4	CCCAGTTACCATAGGGAACA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	56.2	56.5	2252-2255 bp	1.0	54.1
ndhA_p5	ACCCAGTTACCATAGGGAAC	TGGGAGAAGTTGATCGTTGA	50.0	45.0	56.2	56.5	2253-2256 bp	1.0	54.1