Hotspot

infA

LSC · 76391-76714 · 324 bp

Back to Calamagrostis

Pi 0.0036

MarkerSeek score 36.90

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 324
variable_sites: 2
pi: 0.0036
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	TCGCTTGAATTTTTGAACTTCGA	GCCCGAATTCTATCTCGACC	34.8	55.0	57.8	57.9	438 bp	1.0	54.5
infA_p2	TGGAGAAAATAAGTCTCTTCGCT	GCCCGAATTCTATCTCGACC	39.1	55.0	57.6	57.9	456 bp	1.0	52.6
infA_p3	CGCTTGAATTTTTGAACTTCGA	GCCCGAATTCTATCTCGACC	36.4	55.0	56.5	57.9	437 bp	1.0	51.6
infA_p4	TCGCTTGAATTTTTGAACTTCG	GCCCGAATTCTATCTCGACC	36.4	55.0	56.5	57.9	438 bp	1.0	51.6
infA_p5	TCGCTTGAATTTTTGAACTTCGA	CGCCTATACTGCCCGAATTC	34.8	55.0	57.8	58.6	448 bp	1.0	50.5