Hotspot

rpl32-trnL-UAG

SSC · 105770-106407 · 638 bp

Back to Calamagrostis

Pi 0.00344
MarkerSeek score 52.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
638
variable_sites
4
pi
0.00344
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GTTTTTCGGGGCAACAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.0 58.0 731-739 bp 1.0 81.2
rpl32-trnL-UAG_p2 TTTTTCGGGGCAACAACAAC TTCGAACCGAGATGCTTGAG 45.0 50.0 58.0 58.0 730-738 bp 1.0 81.2
rpl32-trnL-UAG_p3 GTTTTTCGGGGCAACAACAA GGATTCGAACCGAGATGCTT 45.0 50.0 58.0 58.1 734-742 bp 1.0 80.7
rpl32-trnL-UAG_p4 TTTTTCGGGGCAACAACAAC GGATTCGAACCGAGATGCTT 45.0 50.0 58.0 58.1 733-741 bp 1.0 80.7
rpl32-trnL-UAG_p5 GGGGCAACAACAACAAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 57.9 58.0 724-732 bp 1.0 80.5