Hotspot

ndhF-rpl32

SSC · 104682-105583 · 902 bp

Back to Calamagrostis

Pi 0.00298
MarkerSeek score 52.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
902
variable_sites
5
pi
0.00298
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 1006-1013 bp 1.0 41.0
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 979-986 bp 1.0 41.0
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 980-987 bp 1.0 41.0
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 981-988 bp 1.0 41.0
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 982-989 bp 1.0 41.0