Hotspot

rps11-rpl36

LSC · 75992-76170 · 179 bp

Back to Calamagrostis

Pi 0.014

MarkerSeek score 52.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 4
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TTCTACGTGCACTTTTCCGT	GCGTGGACGAATTAGAGTCA	45.0	50.0	57.8	57.7	334 bp	1.0	76.9
rps11-rpl36_p2	TTCTACGTGCACTTTTCCGT	GGCGTGGACGAATTAGAGTC	45.0	55.0	57.8	58.4	335 bp	1.0	75.6
rps11-rpl36_p3	TTCTACGTGCACTTTTCCGT	CGTAGGCGTGGACGAATTAG	45.0	55.0	57.8	58.5	339 bp	1.0	74.9
rps11-rpl36_p4	CGCACTTTCTTACGTGAACC	GCGTGGACGAATTAGAGTCA	50.0	50.0	57.4	57.7	281 bp	1.0	74.4
rps11-rpl36_p5	CGCACTTTCTTACGTGAACC	GGCGTGGACGAATTAGAGTC	50.0	55.0	57.4	58.4	282 bp	1.0	73.0