Hotspot

rpl33-rps18

LSC · 65555-65836 · 282 bp

Back to Calamagrostis

Pi 0.00473

MarkerSeek score 34.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 2
pi: 0.00473
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACGACTCATCACGAAATAAAGA	AGAAAAGGTTGTTTAGATGTATACA	36.4	28.0	55.6	54.5	333-342 bp	1.0	40.9
rpl33-rps18_p2	ACGACTCATCACGAAATAAAGA	TTAAGAAAAGGTTGTTTAGATGT	36.4	26.1	55.6	52.2	336-345 bp	1.0	40.9
rpl33-rps18_p3	ACGACTCATCACGAAATAAAGAA	AGAAAAGGTTGTTTAGATGTATACA	34.8	28.0	56.3	54.5	333-342 bp	1.0	40.9
rpl33-rps18_p4	ACGACTCATCACGAAATAAAGA	AAGAAAAGGTTGTTTAGATGTATACA	36.4	26.9	55.6	55.2	334-343 bp	1.0	40.9
rpl33-rps18_p5	CATACGACTCATCACGAAAT	AGAAAAGGTTGTTTAGATGTATACA	40.0	28.0	53.3	54.5	336-345 bp	1.0	40.9