Hotspot

psaJ-rpl33

LSC · 64923-65353 · 431 bp

Back to Calamagrostis

Pi 0.00117

MarkerSeek score 54.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 1
pi: 0.00117
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TATCTCTCTGTAGCACCCGT	GAAATCCCCGTCGATTCCTC	50.0	55.0	57.3	58.1	631-634 bp	1.0	73.6
psaJ-rpl33_p2	TATCTCTCTGTAGCACCCGT	GGCACCTTTTCGAACACAAC	50.0	50.0	57.3	58.2	608-611 bp	1.0	73.4
psaJ-rpl33_p3	TATCTCTCTGTAGCACCCGT	CGATTCCTCATTGGCACCTT	50.0	50.0	57.3	58.2	620-623 bp	1.0	72.7
psaJ-rpl33_p4	ATCTCTCTGTAGCACCCGTG	GAAATCCCCGTCGATTCCTC	55.0	55.0	58.9	58.1	630-633 bp	1.0	72.3
psaJ-rpl33_p5	ATCTCTCTGTAGCACCCGTG	GGCACCTTTTCGAACACAAC	55.0	50.0	58.9	58.2	607-610 bp	1.0	72.1